IDENTIFICATION AND PURIFICATION OF PYRONIN AND RHODAMINE DYES

被引：25

作者：

HOROBIN, RW

MURGATROYD, LB

机构：

[1] Department of Human Biology and Anatomy, The University of Sheffield

来源：

STAIN TECHNOLOGY | 1969年 / 44卷 / 06期

基金：

英国医学研究理事会;

关键词：

D O I：

10.3109/10520296909063370

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The purity of 27 commercial pyronin and rhodamine samples was studied by thin-layer chromatography and visible spectroscopy. Seven different red dyes were detected and separated. The chemical identities of 6 of these were established by nuclear magnetic resonance spectroscopy. The identities of samples sold as rhodamine B and rhodamine 6G were as labelled 8 out of 9 times, the pyronin (G)Y samples were as labelled 5 out of 8 times and the 10 samples sold as acridine red, pyronin B, rhodamine 36 and rhodamine S were always incorrectly labelled. The dextrin and salt contents of the dyes were determined by solvent extraction of the dye with dry methanol or ethanol. Amounts of dextrin and salt varied from none to nearly 90%. Practical methods for identification, separation of coloured components and removal of dextrin and salt are given. © 1969 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：297 / +

页数：1

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