IMPROVED MICROSCOPIC OBSERVATION OF MAMMALIAN-CELLS ON MICROCARRIERS BY FLUORESCENT STAINING

被引：18

作者：

NIKOLAI, TJ

PESHWA, MV

GOETGHEBEUR, S

HU, WS

机构：

[1] Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, 55455, MN

来源：

CYTOTECHNOLOGY | 1991年 / 5卷 / 02期

关键词：

MICROCARRIERS; VIABILITY; FLUORESCENCE; ETHIDIUM BROMIDE;

D O I：

10.1007/BF00365430

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Many microcarriers used for the cultivation of animal cells do not allow for convenient microscopic observation of cell morphology and viability due to their optical properties. Using fluorescent viable stain combining fluorescein diacetate and ethidium bromide, we observed the distribution, morphology and viability of cells on various microcarriers.

引用

页码：141 / 146

页数：6

共 15 条

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