MOLECULAR-CLONING IN ESCHERICHIA-COLI, EXPRESSION, AND NUCLEOTIDE-SEQUENCE OF THE GENE FOR THE ETHYLENE-FORMING ENZYME OF PSEUDOMONAS-SYRINGAE PV PHASEOLICOLA-PK2

被引：46

作者：

FUKUDA, H

OGAWA, T

ISHIHARA, K

FUJII, T

NAGAHAMA, K

OMATA, T

INOUE, Y

TANASE, S

MORINO, Y

机构：

[1] INST PHYS & CHEM RES,WAKO,SAITAMA 35101,JAPAN

[2] KUMAMOTO UNIV,SCH MED,DEPT BIOCHEM,KUMAMOTO 860,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 188卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)91131-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene for the ethylene-forming enzyme of Pseudomonas syringae pv. phaseolicola PK2 was found to be encoded by an indigenous plasmid, designated pPSP1. The gene for the ethylene-forming enzyme was cloned and expressed in Escherichia coli JM109. Nucleotide sequence analysis of the clone revealed an open reading frame that encodes 350 amino acids (mol. wt. 39,444). In a comparison with other proteins, the homology score for the entire amino-acid sequence of the ethylene-forming enzyme of Pseudomonas syringae versus ethylene-forming enzymes from plants and 2-oxoglutarate-dependent dioxygenases was low. However, functionally significant regions are conserved. © 1992.

引用

页码：826 / 832

页数：7

共 24 条

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