MOLECULAR-BASIS AND PCR-DNA TYPING OF THE FYA/FYB BLOOD-GROUP POLYMORPHISM

被引:106
作者
TOURNAMILLE, C [1 ]
KIM, CLV [1 ]
GANE, P [1 ]
CARTRON, JP [1 ]
COLIN, Y [1 ]
机构
[1] INST NATL TRANSFUS SANGUINE,INSERM,U76,F-75015 PARIS,FRANCE
关键词
D O I
10.1007/BF00208965
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The Duffy blood group antigens are carried by the erythrocyte membrane glycoprotein gpD, which has a molecular weight of 35-45 kDa and which has been recently cloned. In this report, we have determined, at the nucleic acid level, the molecular basis for the blood group Fya/Fyb polymorphism. The gpD cDNAs isolated by reverse transcription/polymerase chain reaction (RT-PCR) from Fy(a+b-) and Fy(a-b+) donors differed by only one base susbstitution (G131A) changing Gly to Asp at position 44 of the gpD protein. When expressed in simian Cos-7 cells, the Fy(a+b-) and Fy(a-b+) gpD cDNA produce cell surface proteins that react with the anti-Fya and anti-Fyb antisera, respectively, demonstrating that they represent the FY*A and FY*B alleles of the Duffy blood group locus. The G131A nucleotide substitution has been correlated with a BanI restriction site polymorphism, which has allowed us to develop a method for the DNA typing of the main Duffy blood group antigens, by means of PCR/restriction fragment length polymorphisms.
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页码:407 / 410
页数:4
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