LIMITING DILUTION ANALYSIS OF HUMAN T-CELLS - A USEFUL CLINICAL TOOL

被引：152

作者：

SHARROCK, CEM ^{[1
]}

KAMINSKI, E ^{[1
]}

MAN, S ^{[1
]}

机构：

[1] ROYAL POSTGRAD MED SCH,DEPT IMMUNOL,LONDON W12 0HS,ENGLAND

来源：

IMMUNOLOGY TODAY | 1990年 / 11卷 / 08期

关键词：

D O I：

10.1016/0167-5699(90)90113-N

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Limiting dilution analysis (LDA) provides a practical and simple method for determining the frequency of defined clones of lymphocytes responding to a specific antigen or with a particular effector function. The importance of the technique stems from the fact that it is the only way to assess the immune response in humans, at the level of the cell, in a quantitative manner. In this article Claire Sharrock, Edward Karninski and Stephen Man review the current status of the technique and its applications in human immunology. © 1990.

引用

页码：281 / 286

页数：6

共 40 条

[1]

ALP N, IN PRESS J IMMUNOL M

[2] THE FOREIGN ANTIGEN-BINDING SITE AND T-CELL RECOGNITION REGIONS OF CLASS-I HISTOCOMPATIBILITY ANTIGENS [J].

BJORKMAN, PJ ;

SAPER, MA ;

SAMRAOUI, B ;

BENNETT, WS ;

STROMINGER, JL ;

WILEY, DC .

NATURE, 1987, 329 (6139) :512-518

[3] HUMAN CYTOMEGALOVIRUS-SPECIFIC CYTO-TOXIC T-CELLS - RELATIVE FREQUENCY OF STAGE-SPECIFIC CTL RECOGNIZING THE 72-KD IMMEDIATE EARLY PROTEIN AND GLYCOPROTEIN-B EXPRESSED BY RECOMBINANT VACCINIA VIRUSES [J].

BORYSIEWICZ, LK ;

HICKLING, JK ;

GRAHAM, S ;

SINCLAIR, J ;

CRANAGE, MP ;

SMITH, GL ;

SISSONS, JGP .

JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 168 (03) :919-931

[4] HUMAN CYTOMEGALOVIRUS-SPECIFIC CYTO-TOXIC T-CELLS - THEIR PRECURSOR FREQUENCY AND STAGE SPECIFICITY [J].

BORYSIEWICZ, LK ;

GRAHAM, S ;

HICKLING, JK ;

MASON, PD ;

SISSONS, JGP .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (02) :269-275

[5]

BRETT SJ, 1987, CLIN EXP IMMUNOL, V68, P510

[6] A HYPOTHETICAL MODEL OF THE FOREIGN ANTIGEN-BINDING SITE OF CLASS-II HISTOCOMPATIBILITY MOLECULES [J].

BROWN, JH ;

JARDETZKY, T ;

SAPER, MA ;

SAMRAOUI, B ;

BJORKMAN, PJ ;

WILEY, DC .

NATURE, 1988, 332 (6167) :845-850

[7] DIRECT ESTIMATION OF THE FREQUENCY OF HUMAN CYTO-TOXIC LYMPHOCYTES-T AND THEIR PRECURSORS FOLLOWING INVITRO ALLOSENSITIZATION [J].

BRYAN, DJ ;

PINTO, CE ;

FULLER, TC .

HUMAN IMMUNOLOGY, 1983, 6 (01) :31-44

[8] HIGH-FREQUENCY OF PRECURSORS OF ANOMALOUS KILLER CELLS IN HUMAN PERIPHERAL-BLOOD - EVIDENCE FOR T-CELL REGULATION [J].

BURNS, GF ;

WERKMEISTER, JA ;

TRIGLIA, T .

CELLULAR IMMUNOLOGY, 1984, 89 (01) :202-211

[9] THE EVALUATION OF LIMITING DILUTION ASSAYS [J].

DESTGROTH, SF .

JOURNAL OF IMMUNOLOGICAL METHODS, 1982, 49 (02) :R11-R23

[10] LYMPHOCYTE-T CONTROL OF AUTOREACTIVITY - ANALYSIS WITH HUMAN T-CELL CLONES AND LIMITING DILUTION CULTURE [J].

DEUSCH, K ;

MOEBIUS, U ;

ZUMBUSCHENFELDE, KHM ;

MEUER, SC .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1986, 16 (11) :1433-1438

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