EFFECT OF EDTA ON CYTOKERATIN DETECTION IN THE INNER-EAR

被引：29

作者：

TONNAER, EL

KUIJPERS, W

PETERS, TA

RAMAEKERS, FC

机构：

[1] CATHOLIC UNIV NIJMEGEN,DEPT OTORHINOLARYNGOL,PHILIPS VAN LEYDENLAAN 15,6500 HB NIJMEGEN,NETHERLANDS

[2] CATHOLIC UNIV NIJMEGEN,DEPT PATHOL,6500 HB NIJMEGEN,NETHERLANDS

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1990年 / 38卷 / 08期

关键词：

cytokeratin; EDTA; EGTA; fixation; immunohistochemist ry; inner ear epithelium; unmasking;

D O I：

10.1177/38.8.1694877

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Immunohistochemical studies on the epithelium of the adult inner ear are difficult to perform without decalcification of the bone capsule. In this study, we examined the effect of decalcifying agents on the immunoreactivity of vairous cytokeratin antigens in the cochlear duct epithelium of 2-day-old rats, allowing the comparison of fresh and decalcified specimens. Decalcification of unfixed tissue in a solution containing EDTA or EGTA and polyvinylpyrrolidone, at pH 7.4 and 4°C for a maximum period of 2 days, not only preserved the antigen epitopes but even enhanced the staining intensities in comparison with fresh specimens. This enhancement effect, caused by chelating agents and found to be blocked by prior fixation with acetone, is suggested to be caused by unmasking of the antigenic epitopes.

引用

页码：1223 / 1227

页数：5

共 23 条

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