RETENTION BEHAVIOR OF SOME SYNTHETIC NUCLEOSIDES ON CN, DIOL, AND NH2 PRECOATED HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC PLATES

The retentions of 21 5-substituted deoxyuridine derivatives on cyano, diol, and amino precoated high-performance thin-layer chromatographic plates are determined using adsorption (dichloroethane-methanol and acetontrile-ethylcellosolve mixtures) and reversed-phase (water-methanol mixtures) eluent systems. The retention strengths (potencies) and selectivities are separated by the spectral mapping technique, and the potencies of the solutes are correlated with the physicochemical parameters by using stepwise regression analysis. In aqueous eluents the lipophilicity of the solute determines the retention, i.e. the cyano and diol plates behave as real reversed-phase stationary phases, but some selectivity difference is observed. The low retention capacities and elongated spots make the amino plate unsuitable for the reversed-phase separation of these nucleosides. In the adsorption separation mode, the selectivity of each plate type differs significantly from that of silica. The selectivities of the cyano and diol plates are similar, whereas that of the amino plate differs considerably and also depends on the composition of the eluent.