1-CHLORO-2,4-DINITROBENZENE-MEDIATED IRREVERSIBLE INACTIVATION OF ACIDIC GLUTATHIONE S-TRANSFERASES - INACTIVATION MECHANISM - A SATURATION-TYPE OR SIMPLE 2ND-ORDER KINETIC PROCESS

被引：2

作者：

ADAMS, PA ^{[1
]}

SIKAKANA, CNT ^{[1
]}

机构：

[1] UNIV CAPE TOWN,SCH MED,DEPT MED BIOCHEM,CAPE TOWN 7925,SOUTH AFRICA

来源：

BIOCHEMICAL PHARMACOLOGY | 1992年 / 43卷 / 08期

关键词：

D O I：

10.1016/0006-2952(92)90707-P

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A critical analysis of the inactivation kinetics exhibited by the acidic human glutathione S-transferase (GST) enzymes is presented. Data on the 1-chloro-2,4-dinitrobenzene (CDNB)-facilitated inactivation of human placental GST-pi have been utilized in conjunction with published inactivation data from the literature to answer the following two questions: (a) do the inactivation kinetics deviate significantly from a simple pseudo first-order model? (b) What is the kinetic mechanism of irreversible electrophilic co-substrate-mediated inactivation of human acidic GSTs? Inactivation of human placental GST-pi in the presence of 7-aminocephalosporanic acid. a non-electrophilic non-substrate ligand, is characterized and shown to occur via a process analogous to the second mechanism proposed for CDNB inactivation of the enzyme, namely: pH- and [ligand]-independent solvational inactivation.

引用

页码：1757 / 1760

页数：4

共 19 条

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