USE OF ACRIDINIUM-ESTER-LABELED DNA PROBES FOR IDENTIFICATION OF MYCOBACTERIA IN BACTEC 13A BLOOD CULTURES

被引：6

作者：

EPHRAIM, DA

SPITZER, ED

机构：

[1] SUNY STONY BROOK,DEPT PATHOL,STONY BROOK,NY 11794

[2] SUNY HOSP STONEY BROOK,DEPT LABS,STONY BROOK,NY

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 1994年 / 18卷 / 03期

关键词：

D O I：

10.1016/0732-8893(94)90081-7

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

AccuProbe rests for mycobacteria (Gen-Probe) cannot be performed directly on BACTEC 13A cultures because of interfering substances. This problem can be circumvented by subculturing to BACTEC 12B media. Artifactual chemiluminescence greater than the positive cutoff was seen in 15 of 19 13A cultures containing Mycobacterium avium complex compared with 0 of 19 in 12B subcultures. Of the subcultures, 89% were tested after overnight incubation.

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页码：137 / 139

页数：3

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