DETERMINATION OF ANABOLIC-STEROID 19-NOR-TESTOSTERONE IN BOVINE SERUM BY GC-SIM-MS

Anabolic steroids extracted from bovine serum by means of organic solvents or solid-phase extraction techniques with Ci8 cartridges are not suitable for quantitative analysis by gas chromatography-mass spectrometry (GC-MS); therefore, they were extracted from serum by Extrelut columns (Merck; Germany), eluted with diethyl ether, and purified by a solid- phase extraction technique using amino (NH2) columns. The resulting clean extract was treated with heptafluorobutyric anhydride (HFBA); this formed a derivative suitable for GC-MS analysis. Using selected ion monitoring (SIM), quantities as small as 40 pg/mL can be detected. © Oxford University Press 1992.