PHYSICAL MEASUREMENTS OF LIVER GLUCOCORTICOID RECEPTOR

被引：23

作者：

LITWACK, G ^{[1
]}

CAKE, MH ^{[1
]}

FILLER, R ^{[1
]}

TAYLOR, K ^{[1
]}

机构：

[1] TEMPLE UNIV,SCH MED,DEPT BIOCHEM,PHILADELPHIA,PA 19140

来源：

BIOCHEMICAL JOURNAL | 1978年 / 169卷 / 02期

关键词：

D O I：

10.1042/bj1690445

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Physical measurements were made on the cytosolic form of the liver [3H]dexamethasone receptor. These include a Stoke radius of 3.5 nm, determined by gel filtration, and sedimentation coefficients of 5.1 and 7-8S, by sucrose-density-gradient centrifugation. From these measurements, the following physical properties were calculated: apparent mol.wt. 78000 (the 5.1S form); D(app) 6.1x10-7 cm2.S-1; f/f0 1.25; axial ratio 4.7; these indicate a globular protein. Measurements of sedimentation coefficients of cytosol steroid-receptor complexes previously subjected to various activating conditions gave different values and lead to the conclusion that the mechanism of activation in vitro enabling the steroid-receptor complex to bind to DNA is more complex than simple disaggregation to a uniform size.

引用

页码：445 / 448

页数：4