RAPID AUTOMATIC ANALYSIS OF SUGAR COMPONENTS IN GLYCOPROTEINS .I. HEXOSAMINES

被引：35

作者：

LEE, YC

SCOCCA, JR

MUIR, L

机构：

[1] Department of Biology, The Johns Hopkins University, Baltimore

[2] McCollum-Pratt Institute, The Johns Hopkins University, Baltimore

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 27卷 / 03期

关键词：

D O I：

10.1016/0003-2697(69)90070-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid method is described for the automatic determination of hexosamines in glycoproteins. Hexosamines are separated by cation-exchange chromatography and automatically determined by a neocuproine reducing sugar method. The method is highly reproducible and as little as 1 μg of glucosamine can be measured accurately. © 1969.

引用

页码：559 / &

共 14 条

[1] BOAS NF, 1953, J BIOL CHEM, V204, P553
[2] ION-EXCHANGE SEPARATION AND AUTOMATED ASSAY OF COMPLEX MIXTURES OF AMINO ACIDS AND SOME HEXOSAMINES .2.
BRENDEL, K
STEELE, RS
WHEAT, RW
DAVIDSON, EA
[J]. ANALYTICAL BIOCHEMISTRY, 1967, 18 (01) : 161 - &
[3] ION-EXCHANGE SEPARATION AND AUTOMATED ASSAY OF SOME HEXOSAMINES .I.
BRENDEL, K
ROSZEL, NO
WHEAT, RW
DAVIDSON, EA
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[4] IDENTIFICATION OF AMINO SUGARS
CRUMPTON, MJ
[J]. BIOCHEMICAL JOURNAL, 1959, 72 : 479 - 486
[5] DETERMINATION OF REDUCING SUGAR WITH IMPROVED PRECISION
DYGERT, S
LI, LH
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THOMA, JA
[J]. ANALYTICAL BIOCHEMISTRY, 1965, 13 (03) : 367 - &
[6] SEPARATION AND ESTIMATION OF CHITOSAMINE AND CHONDROSAMINE IN COMPLEX HYDROLYSATES
EASTOE, JE
[J]. NATURE, 1954, 173 (4403) : 540 - 541
[7] SEPARATION ON DOWEX-50 ION EXCHANGE RESIN OF GLUCOSAMINE AND GALACTOSAMINE AND THEIR QUANTITATIVE DETERMINATION
GARDELL, S
[J]. ACTA CHEMICA SCANDINAVICA, 1953, 7 (01): : 207 - 215
[8] DETERMINATION OF HEXOSAMINES
LEE, YC
MONTGOMERY, R
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1961, 93 (02) : 292 - &
[9] RAPID AUTOMATIC ANALYSIS OF SUGAR COMPONENTS OF GLYCOPROTEINS .2. NEUTRAL SUGARS
LEE, YC
MCKELVY, JF
LANG, D
[J]. ANALYTICAL BIOCHEMISTRY, 1969, 27 (03) : 567 - &
[10] LEE YG, UNPUBLISHED RESULTS

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