MOLECULAR AND GENETIC-CHARACTERIZATION OF HUMAN CELL-LINES RESISTANT TO L-ASPARAGINASE AND ALBIZZIIN

被引：21

作者：

ANDRULIS, IL ^{[1
]}

ARGONZA, R ^{[1
]}

CAIRNEY, AEL ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT MED GENET,TORONTO M5S 1A1,ONTARIO,CANADA

来源：

SOMATIC CELL AND MOLECULAR GENETICS | 1990年 / 16卷 / 01期

关键词：

D O I：

10.1007/BF01650480

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human cell lines resistant to l-asparaginase or albizziin were isolated by multistep selection of HT1080 fibrosarcoma and MIA PaCa-2 pancreatic carcinoma cells. Mutants were cross-resistant to both drugs, but more resistant to the drug used for selection. The drug-resistant cell lines expressed elevated levels of asparagine synthetase activity and protein, up to 17-fold over that of the parental cells. Enzyme overproduction was due to gene amplification in the albizziin-resistant cells, whereas increased expression without amplification was observed in l-asparaginase-resistant cells. © 1990 Plenum Publishing Corporation.

引用

页码：59 / 65

页数：7

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