CHANGES IN GROWTH AND PANCREATIC MESSENGER-RNA CONCENTRATIONS DURING POSTNATAL-DEVELOPMENT OF RAT PANCREAS

被引：12

作者：

IOVANNA, JL

DUSETTI, N

CADENAS, B

CALVO, EL

机构：

[1] Departamento de Fisiología, Universidad de Beunos Aires, Buenos Aires

来源：

PANCREAS | 1990年 / 5卷 / 04期

关键词：

Actin; Amylase; Chymo-trypsinogen; Pancreas development; Pancreatic mRNAs; Proinsulin;

D O I：

10.1097/00006676-199007000-00008

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Changes in pancreatic growth and in mRNA concentrations in rat pancreas were monitored by dot-blot hybridization with cloned cDNAs of rat amylase, chymotrypsinogen B, proinsulin I, and actin during the pre-and postnatal period in the rat. Wistar rats were killed at the 18th day of gestation and at the 1st, 10th, 20th, 35th, and 87th day of postnatal life. It was concluded from the ratio of pancreatic weight/body weight that pancreatic growth preceded body growth. Pancreatic protein and total RNA concentration increased 2.9 times during the period studied. All studied mRNAs increased in concentration during the postnatal development period. Messenger RNA for chymotrypsinogen B and proinsulin I exhibited a significant increase after birth, decreased by the 10th day of life, and increased thereafter. For amylase mRNA, no significant changes were observed around birth, a progressive increase occurring thereafter up to the 87th day of life. The mRNA for actin showed a progressive increase between the 18th day of gestation and the 20th postnatal day, after which it remained stable. We concluded that each mRNA showed a singular profile of increase during postnatal development. © 1990 Raven Press Ltd., New York.

引用

页码：421 / 426

页数：6

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