RIBOSOMAL-RNA AND RDNA SEQUENCE ANALYSES

被引：64

作者：

STACKEBRANDT, E ^{[1
]}

LIESACK, W ^{[1
]}

WITT, D ^{[1
]}

机构：

[1] GESELL STRAHLEN & UMWELTFORSCH MBH,INST OKOL CHEM,W-8042 NEUHERBERG,GERMANY

来源：

GENE | 1992年 / 115卷 / 1-2期

关键词：

STREPTOMYCES; PHYLOGENY; SEQUENCE DATA ANALYSIS; TREE TOPOLOGY; PCR; DIAGNOSTIC PROBES; PHENETIC CLUSTERS;

D O I：

10.1016/0378-1119(92)90567-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The potential of ribosomal (r) RNA and the encoding genes (rDNA) to elucidate natural relationships has been dramatically extended by improved sequencing approaches and the application of polymerase chain reaction. Sequence information on 16S and 23S rRNA/DNA from 69 strains of 53 Streptomyces species allows determination of regions that can be used as target sites for diagnostic probes, and for amplification and sequencing primers. To generate phylogenetic trees, sequence similarities are converted into distance values. The topologies of the trees based on different parts of the molecule are compared among each other and to the numerical phenotypic clustering of the strains.

引用

页码：255 / 260

页数：6

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