REGIONALLY SELECTIVE STIMULATION OF MITOGEN-ACTIVATED PROTEIN (MAP) KINASE TYROSINE PHOSPHORYLATION AFTER GENERALIZED SEIZURES IN THE RAT-BRAIN

被引：60

作者：

GASS, P ^{[1
]}

KIESSLING, M ^{[1
]}

BADING, H ^{[1
]}

机构：

[1] HARVARD UNIV, SCH MED, DEPT MICROBIOL & MOLEC GENET, BOSTON, MA 02115 USA

来源：

NEUROSCIENCE LETTERS | 1993年 / 162卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

EPILEPSY; MAP KINASE; TYROSINE PHOSPHORYLATION; IMMUNOCYTOCHEMISTRY; IMMUNOBLOTTING;

D O I：

10.1016/0304-3940(93)90554-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Immunoblot analysis using a phosphotyrosine-specific antibody was performed to investigate tyrosine phosphorylation of the mitogen activated protein (MAP) kinase in the rat brain. Epileptic seizures induced by systemic injection of bicuculline caused a rapid and transient stimulation of MAP kinase tyrosine phosphorylation in hippocampus and somatosensory cortex. This increase in tyrosine phosphorylation was markedly attenuated by the N-methyl-D-aspartate (NMDA) receptor antagonist MK-801. In contrast, in the cerebellum, tyrosine phosphorylation of MAP kinase remained undetectable after bicuculline-induced seizures. These results demonstrate that generalized seizures stimulate tyrosine phosphorylation of MAP kinase in a regionally selective manner.

引用

页码：39 / 42

页数：4

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