ON SOME AUTOLYZED DERIVATIVES OF BOVINE TRYPSIN

被引：63

作者：

MAROUX, S

DESNUELLE, P

机构：

[1] Centre de Biochimie et de Biologie Moléculaire, C.N.R.S., Marseille

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 181卷 / 01期

关键词：

D O I：

10.1016/0005-2795(69)90227-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatography or incubation of dilute solutions of bovine trypsin at 4° and pH 5.5 in the presence of Ca2+ appears to induce almost exclusively the autolytic cleavage of bond Arg105-Val106. Bonds Lys49-Ser50 and Lys131-Ser132 are also found substantially autolyzed in commercial trypsin preparations. Evidence is presented suggesting that trypsin still retain activity after cleavage of bonds Arg105-Val106 and Lys131-Ser132. In contrast, the cleavage of bond Lys49-Ser50, close to residue His46, probably inactivates the enzyme. © 1969.

引用

页码：59 / +

页数：1

共 23 条

[1] CRESTFIELD AM, 1963, J BIOL CHEM, V238, P622
[2] DAVIE EW, 1955, J BIOL CHEM, V212, P515
[3] DELAAGE M., 1965, BIOCHIM BIOPHYS ACTA, V105, P608
[4] SUR LA SEQUENCE N-TERMINALE DU TRYPSINOGENE ET SON ABLATION PENDANT LACTIVATION DE CE ZYMOGENE
DESNUELLE, P
FABRE, C
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1955, 18 (01) : 49 - 57
[5] DIXON GH, 1958, J BIOL CHEM, V233, P1373
[6] GABELOTEAU C, 1957, ARCH BIOCHEM BIOPHYS, V69, P475
[7] FORMATION AND ACTIVATION OF NEOCHYMOTRYPSINOGEN B
GUY, O
ROVERY, M
DESNUELLE, P
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1966, 124 (02) : 402 - +
[8] EVOLUTIONARY SIMILARITIES BETWEEN PANCREATIC PROTEOLYTIC ENZYMES
HARTLEY, BS
BROWN, JR
KAUFFMAN, DL
SMILLIE, LB
[J]. NATURE, 1965, 207 (5002) : 1157 - &
[9] PEPTIDES FROM PEPTIC DIGEST OF BOVINE DIP-TRYPSIN AND DETERMINATION OF DISULFIDE BONDS
HOLEYSOVSKY, V
MESROB, B
TOMASEK, V
MIKES, O
SORM, F
[J]. COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS, 1968, 33 (02) : 441 - +
[10] MAROUX S, 1962, BIOCHIM BIOPHYS ACTA, V56, P202, DOI 10.1016/0006-3002(62)90556-5

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