HISTOCHEMICAL STUDY OF CARBONIC ANHYDRASE IN KIDNEY OF MOUSE, WITH SPECIAL REFERENCE TO INHIBITORY EFFECT OF DIAMOX

With the histochemical method of Häusler, activity of carbonic anhydrase (CAH) in normal mouse kidney was found most concentrated in proximal tubules, while distal tubules showed less intense activity of CAH. Specificity of the reaction was confirmed by complete block of the reaction with either in vivo or in vitro application of Diamox. After in vivo administration of Diamox, even in a large dose, complete block of CAH in sections was detectable for about half an hour. Duration of this blocking effect could be sustained longer by simultaneous administration of sodium bicarbonate, but not when the alkali and Diamox had been given successively. Reduction of the effect of Diamox by acidosis was also confirmed in the mice in a state of artificial acidosis which had been induced by oral administration of ammonium chloride. © 1969, Tohoku University Medical Press. All rights reserved.