IMMOBILIZED GLUCOSE-OXIDASE AND D-AMINO-ACID OXIDASE - CONVENIENT METHOD FOR THE PURIFICATION OF FLAVIN ADENINE-DINUCLEOTIDE AND ITS ANALOGS

被引：15

作者：

MASSEY, V

MENDELSOHN, LD

机构：

[1] Department of Biological Chemistry, The University of Michigan, Ann Arbor

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(79)90198-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The preparation of flavin adenine dinucleotide-affinity columns employing glucose oxidase and d-amino acid oxidase covalently linked to Sepharose-4B is described. Both immobilized enzymes have very good long-term stabilities, retaining at least half of their original flavin adenine dinucleotide-binding capacity over 11 months. In both cases pure flavin adenine dinucleotide is obtained readily in good yields. © 1979.

引用

页码：156 / 159

页数：4

共 17 条

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