ISOLATION AND CHARACTERIZATION OF BLEOMYCIN-RESISTANT CLONES OF CHO CELLS

被引：18

作者：

BRABBS, S ^{[1
]}

WARR, JR ^{[1
]}

机构：

[1] UNIV YORK,DEPT BIOL,HESLINGTON YO1 5DD,YORKSHIRE,ENGLAND

来源：

GENETICAL RESEARCH | 1979年 / 34卷 / 03期

关键词：

D O I：

10.1017/S0016672300019509

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Five clones of Chinese hamster ovary cells with increased resistance to bleomycin have been isolated following ethylmethanesulphonate mutagenesis. Resistance was stable in three of the clones, but unstable in the other two. One of the stably resistant clones was cross resistant to unrelated drugs, and in contrast to the parental cells, its response to bleomycin was potentiated by tween 80. These two observations suggested a membrane alteration in the resistant clone. There was no significant difference in bleomycin-inactivating enzyme activity between the parental and resistant clones. © 1979, Cambridge University Press. All rights reserved.

引用

页码：269 / 279

页数：11

共 25 条

[1]

BLUM RH, 1973, CANCER, V31, P903, DOI 10.1002/1097-0142(197304)31:4<903::AID-CNCR2820310422>3.0.CO

[2]

2-N

[3]

CROOKE ST, 1976, J MED, V7, P333

[4] RESISTANCE OF CULTURED HUMAN FIBROBLASTS AND OTHER CELLS TO PURINE AND PYRIMIDINE ANALOGS IN RELATION TO MUTAGENESIS DETECTION [J].

DEMARS, R .

MUTATION RESEARCH, 1974, 24 (03) :335-364

[5]

HITTELMAN WN, 1974, CANCER RES, V34, P3433

[6] GENETICS OF SOMATIC MAMMALIAN CELLS - LINKAGE STUDIES WITH HUMAN-CHINESE HAMSTER CELL HYBRIDS [J].

KAO, FT ;

PUCK, TT .

NATURE, 1970, 228 (5269) :329-+

[7] GENETICS OF SOMATIC MAMMALIAN CELLS .7. INDUCTION AND ISOLATION OF NUTRITIONAL MUTANTS IN CHINESE HAMSTER CELLS [J].

KAO, FT ;

PUCK, TT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 60 (04) :1275-+

[8] DRUG-RESISTANCE AND MEMBRANE ALTERATION IN MUTANTS OF MAMMALIAN-CELLS [J].

LING, V .

CANADIAN JOURNAL OF GENETICS AND CYTOLOGY, 1975, 17 (04) :503-515

[9]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[10]

MAYAKI M, 1975, CANCER RES, V35, P2015

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