CENTRIOLE DECILIATION ASSOCIATED WITH THE EARLY RESPONSE OF 3T3 CELLS TO GROWTH-FACTORS BUT NOT TO SV40

被引:97
作者
TUCKER, RW
SCHER, CD
STILES, CD
机构
[1] CHILDRENS HOSP MED CTR, TUMOR BIOL LAB, BOSTON, MA 02115 USA
[2] CHILDRENS HOSP MED CTR, DIV MED ONCOL, BOSTON, MA 02115 USA
[3] CHILDRENS HOSP MED CTR, DIV HEMATOL ONCOL, BOSTON, MA 02115 USA
[4] CHILDRENS HOSP MED CTR, DEPT PEDIAT, BOSTON, MA 02115 USA
[5] SIDNEY FARBER CANC INST, TUMOR BIOL LAB, GRP W, BOSTON, MA 02115 USA
[6] SIDNEY FARBER CANC INST, DEPT MICROBIOL & MOLEC GENET, BOSTON, MA 02115 USA
[7] HARVARD UNIV, SCH MED, BOSTON, MA 02115 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0092-8674(79)90219-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
BALB/c-3T3 cells which are growth-arrested by high cell density or low serum have ciliated, unduplicated centrioles. Stimulation of these quiescent cells by serum is associated with a rapid (within 1-2 hr) deciliation of the centriole, followed by reciliation within 6-10 hr. This transient deciliation of the centriole is induced by the platelet-derived growth factor (PDGF) component of serum. The cells treated with PDGF became competent to replicate their DNA; most PDGF treated cells, however, did not progress from Go toward S phase unless they were incubated with the platelet-poor plasma component of serum. Addition of CaCl2 or Fibroblast Growth Factor to the media mimicked PDGF by producing both centriole deciliation and competence to replicate DNA. In fact, over a range of concentrations of each of these factors, only doses which produced centriole deciliation were capable of producing competence for DNA synthesis. Plasma alone or factors such as Multiplication Stimulating Activity produced neither centriole deciliation nor competence; these agents were, however, required for the optimum progression of competent cells into DNA synthesis. In contrast, infection with SV40 induced host cell DNA synthesis without an initial transient deciliation of the centriole. Thus while growth factors may have to induce centriole deciliation for 3T3 cells to synthesize DNA, abortive transformation by SV40 overrides this requirement. © 1979.
引用
收藏
页码:1065 / 1072
页数:8
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