QUANTITATION OF PLASMA FACTOR-XIIIA ACTIVITY USING FIBRIN-COATED MICROSCOPIC LATEX BEADS

被引：11

作者：

RETZINGER, GS ^{[1
]}

COOK, BC ^{[1
]}

SMITH, RE ^{[1
]}

MCGINNIS, MC ^{[1
]}

机构：

[1] MED COLL WISCONSIN,DEPT MED,MILWAUKEE,WI 53226

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 195卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90288-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Following exposure to thrombin, monodisperse microscopic polystyrene-divinylbenzene beads coated with a mixed film of lecithin and fibrinogen aggregate as a consequence of interbead fibrin polymerization. These bead aggregates rapidly dissociate in 5 m urea. Treatment of aggregates with factor XIIIa results in a dosedependent decrease of the rate of aggregate dissociation in urea. The rate of disaggregation is readily quantitated by turbidimetry. We have exploited this phenomenon to develop a rapid and sensitive method for quantitating factor XIIIa activity in plasma. Using 20 μl of human plasma the method measures the activity of factor XIIIa to a level of 0.03 U ml-1 with a precision of ±5%. © 1991.

引用

页码：18 / 23

页数：6

共 23 条

[1]

FIBRIN STABILIZING FACTOR (FACTOR 13) [J].