FACTORS AFFECTING THE LINE WIDTHS AND SIGNAL-TO-NOISE RATIOS OF THE C-13 CP/MAS SPECTRA OF PROTEINS

被引：8

作者：

BELTON, PS

GIL, AM

TANNER, SF

机构：

[1] AFRC Institute of Food Research, Norwich Laboratory, Norwich, NR47UA, Norwich Research Park, Colney

[2] Joint Department of Physics, Institute of Cancer Research and Royal Marsden Hospital, Sutton, Surrey

来源：

MAGNETIC RESONANCE IN CHEMISTRY | 1993年 / 31卷 / 11期

关键词：

CP/MAS SPECTRA; LINE WIDTHS; SIGNAL-TO-NOISE RATIOS; PROTEINS;

D O I：

10.1002/mrc.1260311109

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Typically the C-13 CP/MAS spectra of proteins have low signal-to-noise ratios and broad lines, which considerably degrade the value of such spectra. The origins of these effects were examined using a model protein, lysozyme. The effects of hydration, relaxation time and crystallinity were systematically investigated. It is concluded that low signal-to-noise ratios are the result of the typically low density of protein preparations and not an intrinsic magnetic resonance phenomenon, and that line broadening is principally the result of poor molecular packing in noncrystalline protein preparations.

引用

页码：1001 / 1007

页数：7

共 32 条

[1] CROSS POLARIZATION AND MAGIC ANGLE SAMPLE SPINNING NMR-SPECTRA OF MODEL ORGANIC-COMPOUNDS .3. EFFECT OF THE C-13-H-1 DIPOLAR INTERACTION ON CROSS POLARIZATION AND CARBON-PROTON DEPHASING [J].