A NOSOCOMIAL OUTBREAK DUE TO NONENCAPSULATED HAEMOPHILUS-INFLUENZAE - ANALYSIS OF PLASMIDS CODING FOR ANTIBIOTIC-RESISTANCE

被引:7
作者
ANDERSON, JR [1 ]
SMITH, MD [1 ]
KIBBLER, CC [1 ]
HOLTON, J [1 ]
SCOTT, GM [1 ]
机构
[1] UCL HOSP, DEPT CLIN MICROBIOL, LONDON WC1E 6AU, ENGLAND
关键词
NOSOCOMIAL INFECTION; HAEMOPHILUS-INFLUENZAE PLASMIDS; ANTIBIOTIC RESISTANCE;
D O I
10.1016/0195-6701(94)90064-7
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
An outbreak of infections with non-encapsulated Haemophilus influenzae, resistant to ampicillin, chloramphenicol, sulphonamide and tetracycline involved 13 elderly patients and three nurses on acute admission and care of the elderly wards. Thirty-two isolates were found to be indistinguishable on analysis of biotype, antibiogram, serotype and major outer membrane proteins (MOMP). Plasmids could not be identified in the original isolates but after mating with a Rec A H. influenzae recipient, the resultant transconjugates were found to harbour either a 72 kilobase pair (kB) plasmid coding for resistance to chloramphenicol, ampicillin, sulphonamide and tetracycline or a 65 kB plasmid coding for resistance to chloramphenicol, ampicillin and sulphonamide. Both plasmids yielded virtually indistinguishable restriction digest patterns. This suggests that the tetracycline resistance gene (Tc gene) is a non-essential component of one basic plasmid responsible for the multiple antibiotic resistances seen in the strains recovered during the outbreak. This illustrates the value of plasmid profiles to compare strains of non-encapsulated H. influenzae, and suggests that plasmid restriction enzyme analysis is critical. © 1994.
引用
收藏
页码:17 / 27
页数:11
相关论文
共 23 条
[1]   PLASMID-TO-CHROMOSOME GENE-TRANSFER IN HAEMOPHILUS-INFLUENZAE DURING GROWTH [J].
BALGANESH, M ;
ARRIGONI, L ;
SETLOW, JK .
JOURNAL OF BACTERIOLOGY, 1986, 168 (01) :458-459
[2]   MOLECULAR EPIDEMIOLOGY OF MULTIPLY RESISTANT HAEMOPHILUS-INFLUENZAE TYPE-B IN DAY-CARE-CENTERS [J].
CAMPOS, J ;
GARCIATORNEL, S ;
MUSSER, JM ;
SELANDER, RK ;
SMITH, AL .
JOURNAL OF INFECTIOUS DISEASES, 1987, 156 (03) :483-489
[3]  
DIMOPOULOU ID, 1992, J INFECT DIS, V165, P1069, DOI 10.1093/infdis/165.6.1069
[4]   CROSS-INFECTION BY NONENCAPSULATED HAEMOPHILUS-INFLUENZAE [J].
GOUGH, J ;
KRAAK, WAG ;
ANDERSON, EC ;
NICHOLS, WW ;
SLACK, MPE ;
MCGHIE, D .
LANCET, 1990, 336 (8708) :159-160
[5]  
GRINSTED J, 1988, METHODS MICROBIOLOGY, V21
[6]  
HARDY K, 1986, ASPECTS MICROBIOLOGY, V4
[7]   RAPID PROCEDURE FOR DETECTION AND ISOLATION OF LARGE AND SMALL PLASMIDS [J].
KADO, CI ;
LIU, ST .
JOURNAL OF BACTERIOLOGY, 1981, 145 (03) :1365-1373
[8]  
KILLIAN M, 1976, J GEN MICROBIOL, V93, P9
[9]   R-FACTOR INVOLVEMENT IN A LOCAL OUTBREAK OF AMPICILLIN-RESISTANT HEMOPHILUS-INFLUENZAE INFECTIONS [J].
MALMVALL, BE ;
BRANEFORSHELANDER, P .
SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES, 1978, 10 (01) :53-56
[10]   PLASMID RECOMBINATION IN HEMOPHILUS-INFLUENZAE [J].
MCCARTHY, D .
JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (04) :577-596