IMMUNOHISTOCHEMICAL INVESTIGATION IN ODONTOGENIC MYXOMA

Three odontogenic myxomas are described immunohistochemically by a panel of poly- and monoclonal antibodies to characterize this tumor type. Three types of odontogenic myxoma cells were discriminated: spindle cells, stellate cells and hyaline cells. Neoplastic cells of myxomas were positively stained for transferrin, ferritin, alpha-1-antichymotrypsin (alpha-1-ACT), alpha-1-antitrypsin (alpha-1-AT), S-100 protein and vimentin; however, neuron specific enolase (NSE), S-100 alpha-subunit, S-100 beta-subunit, Factor VIII-related antigen (FVIII-AG) and cytokeratin (CK1) were negative. Spindle cells were positive for transferrin, ferritin, alpha-1-ACT, alpha-1-AT, S-100 protein and vimentin. Stellate cells were strongly positive for transferrin, alpha-1-AT, S-100 protein and vimentin. Hyaline cells reacted with alpha-1-ACT and alpha-1-AT. Myxomatous matrix showed negative reaction for all the antibodies used. These results have confirmed that odontogenic myxoma is a tumor of a dual fibroblastic-histiocytic origin.