CHARACTERIZATION OF OUTER-MEMBRANE AND SECRETED PROTEINS OF LEPTOSPIRA-INTERROGANS SEROVAR POMONA

被引:66
作者
ZUERNER, RL
KNUDTSON, W
BOLIN, CA
TRUEBA, G
机构
[1] USDA, ANIM PLANT HLTH INSPECT SERV, NATL VET SERV LAB, AMES, IA 50010 USA
[2] SANOFI ANIM HLTH INC, LENEXA, KS 66215 USA
[3] IOWA STATE UNIV SCI & TECHNOL, DEPT VET MICROBIOL, AMES, IA 50011 USA
关键词
LEPTOSPIRA-INTERROGANS; OUTER MEMBRANE; SECRETED PROTEINS; ANTIGENS;
D O I
10.1016/0882-4010(91)90014-2
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Outer membrane and secreted proteins were isolated from Leptospira interrogans serovar pomona and characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis, immunoblot and radioimmunoprecipitation techniques. The L. interrogans outer membranes were extracted with Triton X-114 and contained several proteins. The major cellular protein with a molecular mass of 31 kDa was associated exclusively with the L. interrogans outer membrane. Using a whole cell immunoprecipitation method, five hydrophobic, Triton X-114 extractable proteins (22, 26, 31, 36 and 42 kDa) were exposed on the surface of L. interrogans. The 31 kDa protein was heat labile and was a potent antigen in animals experimentally infected with L. interrogans serovar pomona. Several proteins were secreted by L. interrogans including a 60 kDa protein tentatively identified as the L. interrogans hemolysin. © 1991.
引用
收藏
页码:311 / 322
页数:12
相关论文
共 33 条
[1]  
ALEXANDER AD, 1956, P SOC EXP BIOL MED, V91, P205, DOI 10.3181/00379727-91-22214
[2]   ELECTRON MICROSCOPY OF IMMUNE DISRUPTION OF LEPTOSPIRES - ACTION OF COMPLEMENT AND LYSOZYME [J].
ANDERSON, DL ;
JOHNSON, RC .
JOURNAL OF BACTERIOLOGY, 1968, 95 (06) :2293-&
[3]   L-TRANS-EPOXYSUCCINYL-LEUCYLAMIDO(4-GUANIDINO)BUTANE (E-64) AND ITS ANALOGS AS INHIBITORS OF CYSTEINE PROTEINASES INCLUDING CATHEPSINS B, H AND L [J].
BARRETT, AJ ;
KEMBHAVI, AA ;
BROWN, MA ;
KIRSCHKE, H ;
KNIGHT, CG ;
TAMAI, M ;
HANADA, K .
BIOCHEMICAL JOURNAL, 1982, 201 (01) :189-198
[4]   PROTEINASE INHIBITION, IMMUNOGLOBULIN-BINDING PROTEINS AND A NOVEL ANTIMICROBIAL PRINCIPLE [J].
BJORCK, L .
MOLECULAR MICROBIOLOGY, 1990, 4 (09) :1439-1442
[5]  
BOLIN CA, 1989, AM J VET RES, V50, P2004
[6]  
BOLIN CA, 1989, AM J VET RES, V50, P161
[7]  
BORDIER C, 1981, J BIOL CHEM, V256, P1604
[8]  
BORGPETERSEN C, 1971, TROP GEOGR MED, V23, P282
[9]   IMMUNOGENIC INTEGRAL MEMBRANE-PROTEINS OF BORRELIA-BURGDORFERI ARE LIPOPROTEINS [J].
BRANDT, ME ;
RILEY, BS ;
RADOLF, JD ;
NORGARD, MV .
INFECTION AND IMMUNITY, 1990, 58 (04) :983-991
[10]   ANTIGENS RECOGNIZED BY THE HUMAN IMMUNE-RESPONSE TO INFECTION WITH LEPTOSPIRA-INTERROGANS SEROVAR HARDJO [J].
CHAPMAN, AJ ;
ADLER, B ;
FAINE, S .
JOURNAL OF MEDICAL MICROBIOLOGY, 1988, 25 (04) :269-278