PULSED MAGNETIC-FIELD EFFECTS ON CALCIUM SIGNALING IN LYMPHOCYTES - DEPENDENCE ON CELL STATUS AND FIELD INTENSITY

The effect of 3-Hz, monopolar, quasi-rectangular magnetic field pulses on Ca-45(2+) uptake in resting and mitogen-treated rat thymic lymphocytes was evaluated. A 30-min, non-thermal exposure to the pulsed magnetic field (B(peak) = 6.5 mT, E(max) = 0.69 mV/cm, J(max) = 2.6 muA/CM2) reduced Concanavalin A-induced Ca-45(2+) uptake by 45%. It was observed that (i) the induction of the 3-Hz field response dependend on Ca2+ signal transduction activation; (ii) the response direction (stimulation or inhibition) depended on the level of lymphocyte mitogen responsiveness, and (iii) the field response magnitude increased with increasing magnetic field flux densities (B(peak) = 0, 1.6, 6.5 and 28 mT). Our results demonstrate field effects at B(max) nearly 10(4) greater than that of the average human environment for low-frequency magnetic fields and they are consistent with the independent results from other 3-Hz pulsed magnetic field studies with lymphocytes.