PHOSPHORYLATION STATE AND DNA-BINDING ACTIVITY OF C-JUN DEPEND ON THE INTRACELLULAR CONCENTRATION OF BINDING-SITES

被引：30

作者：

PAPAVASSILIOU, AG ^{[1
]}

CHAVRIER, C ^{[1
]}

BOHMANN, D ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB,DIFFERENTIAT PROGRAMME,POSTFACH 102209,MEYERHOFSTR 1,W-6900 HEIDELBERG,GERMANY

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 23期

关键词：

TRANSCRIPTION FACTOR-AP-1; PROTEIN PHOSPHORYLATION; GENE REGULATION; PROTOONCOGENE;

D O I：

10.1073/pnas.89.23.11562

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The DNA-binding activity of c-Jun expressed in eukaryotic cells was found to be markedly enhanced if the intracellular concentration of binding sites for this transcription factor was increased by cotransfection of specific plasmid DNA. Dephosphorylation experiments, phosphate mapping studies, and mutational analysis indicate that phosphorylation of a cluster of serine and threonine residues situated in close proximity to the DNA-binding domain is responsible for the observed adaptation of c-Jun activity to the intracellular concentration of accessible target sites.

引用

页码：11562 / 11565

页数：4

共 11 条

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