CHROMOSOMAL-POLYMORPHISM OF THE YEAST YARROWIA-LIPOLYTICA AND RELATED SPECIES - ELECTROPHORETIC KARYOTYPING AND HYBRIDIZATION WITH CLONED GENES

被引：32

作者：

NAUMOVA, E

NAUMOV, G

FOURNIER, P

NGUYEN, HV

GAILLARDIN, C

机构：

[1] INST NATL AGRON PARIS GRIGNON,CTR BIOTECHNOL AGRO-IND,INRA,CNRS,F-78850 THIVERVAL GRIGNON,FRANCE

[2] STATE INST GENET & SELECT IND MICROORGANISMS,MOSCOW 113545,RUSSIA

来源：

CURRENT GENETICS | 1993年 / 23卷 / 5-6期

关键词：

KARYOTYPE; YARROWIA-LIPOLYTICA; CHROMOSOME POLYMORPHISM; HYBRIDIZATION;

D O I：

10.1007/BF00312633

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Significant differences in electrophoretic karyotyping patterns were found among 27 strains of Y. lipolytica. Twenty-one of these strains were classified into four groups of similar karyotypes while six strains showed unique karyotypes. Chromosomal DNAs of different strains were hybridized with cloned genes of Y lipolytica (URA3, LEU2, ARS18 and ARS68), which revealed four different bands in most strains. We conclude that the haploid chromosome number of Y lipolytica is at least four, and possibly five or six. Electrophoretic karyotyping and hybridization with cloned genes of Y lipolytica provided evidence of a large divergence between Y lipolytica and related species of Saccharomycopsis, Endomycopsella and Endomyces.

引用

页码：450 / 454

页数：5

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