A GENETIC-ANALYSIS OF THE ADENINE PHOSPHORIBOSYL TRANSFERASE LOCUS IN CHINESE-HAMSTER V79-AP4 CELLS - RELEVANCE TO MUTAGENESIS STUDIES

被引：4

作者：

COLELLA, CM ^{[1
]}

SIMI, S ^{[1
]}

VANBOXEL, T ^{[1
]}

TALARICO, D ^{[1
]}

DELLAVALLE, G ^{[1
]}

CARROZZA, ML ^{[1
]}

FRATTA, D ^{[1
]}

MARIANI, T ^{[1
]}

PIRAS, A ^{[1
]}

SIMILI, M ^{[1
]}

RAINALDI, G ^{[1
]}

机构：

[1] DIPARTIMENTO GENET & MICROBIOL, I-27100 PAVIA, ITALY

来源：

MUTATION RESEARCH | 1988年 / 202卷 / 01期

关键词：

D O I：

10.1016/0027-5107(88)90181-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The chromosomal location of the autosomal locus aprt has been investigated in the permanent Chinese hamster cell line V79-AP4 by standard somatic cell genetics methodologies. Aprt is functionally dizygous in V79-AP4 and the 2 alleles map on 2 chromosome 3 homologs, in agreement with the chromosome assignment of the gene in Chinese hamster primary cells. Chromosome G-banding and a Southern blot analysis of V79-AP4 DNA, using as a probe the cloned Chinese hamster aprt gene, have not revealed any structural alteration at either of the 2 aprt alleles. One of the chromosomes 3 has, however, a terminal deletion in its long arm and is therefore morphologically marked. These findings could make V79-AP4 an interesting cell system for the study of mutational mechanisms at the aprt locus in Chinese hamster.

引用

页码：185 / 192

页数：8

共 36 条

[1] GENE-MAPPING AND LINKAGE ANALYSIS IN CHINESE-HAMSTER - ASSIGNMENT OF THE GENES FOR APRT, LDHA, IDH2, AND GAA TO CHROMOSOME-3 [J].