RELEASE OF MEMBRANE CONSTITUENTS FOLLOWING POLYETHYLENE-GLYCOL TREATMENT OF HEP-2 CELLS

被引：11

作者：

MCCAMMON, JR

FAN, VSC

机构：

[1] Department of Microbiology and Immunology, Schools of Medicine and Dentistry, University of Louisville, Louisville

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 551卷 / 01期

关键词：

(HEp-2 cell); Cell fusion; Membrane fragment; Polyethylene glycol;

D O I：

10.1016/0005-2736(79)90353-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HEp-2 [human larynx carcinoma] cell monolayers were treated with 40% polyethylene glycol for 5 min which resulted in fusion during the subsequent incubation period. A loss of cell membrane components was detected in the polyethylene glycol-treated and phosphate buffer/saline-treated control cells; however, the polyethylene glycol-treated cells released nearly twice the amount of [14C]acetate-labeled material and [3H]glycerol-labeled lipids into culture fluids than the control cells. The polyethylene glycol-treated cells released only .apprx. 1/2 the amount of protein, glycoprotein and glycolipid as the control cells. Apparently polyethylene glycol exerts a differential mode of action against cell surface components and causes the treated cells to release membrane components rich in lipids but relatively low in protein and carbohydrate-containing components.

引用

页码：67 / 73

页数：7

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