PURIFICATION AND CHARACTERIZATION OF A 43-KDA PROTEASE OF BACTEROIDES-GINGIVALIS

被引：22

作者：

FUJIMURA, S

NAKAMURA, T

机构：

[1] Department of Oral Microbiology, Matsumoto

[2] Dental College, Shiojiri, Hiro'oka

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1990年 / 5卷 / 06期

关键词：

Bacteroides gingivalis; protease; protein purification;

D O I：

10.1111/j.1399-302X.1990.tb00441.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

From the culture supernatant of Bacteroides gingivalis ATCC 33277, a thiol protease was purified to homogeneity by fractional ammonium sulfate precipitation, ion‐exchange chromatography, gel filtration, and isoelectric focusing. Its molecular weight was 43 kDa and showed similar enzymatic properties to a 300‐kDa protease that was previously characterized. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：360 / 362

页数：3

共 13 条

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