IDENTIFICATION AND DEVELOPMENTAL EXPRESSION OF SRC+ MESSENGER-RNAS IN XENOPUS-LAEVIS

Alternative splicing of src mRNA has been demonstrated in several vertebrate species to yield a neuron-specific form of src protein termed pp60+. The function of pp60+ is unknown. The early developmental expression pattern of src+ RNA has not been previously examined. We have identified and characterized src+ transcripts corresponding to the two src genes in Xenopus laevis using a reverse transcription/polymerase chain reaction ( RT PCR) method. Both Xenopus pp60+ proteins have a 5-amino-acid insert in contrast to the 6-amino-acid insert in fish, birds, and mammals. Src+ mRNA first appears in neural plate stage Xenopus embryos, after neural induction signaling events but prior to neural differentiation. Analysis of dissected neural plate stage embryos showed that src+ mRNAs are localized to the neural plate. These findings suggest that pp60+ may play a role in elaboration of neuron structure. © 1992.