PURIFICATION AND CRYSTALLIZATION OF HUMAN CYCLIN-DEPENDENT KINASE-2

被引：53

作者：

ROSENBLATT, J

DEBONDT, H

JANCARIK, J

MORGAN, DO

KIM, SH

机构：

[1] UNIV CALIF BERKELEY, DEPT CHEM, BERKELEY, CA 94720 USA

[2] LAWRENCE BERKELEY LAB, BERKELEY, CA 94720 USA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 230卷 / 04期

关键词：

CELL CYCLE; CYCLIN DEPENDENT KINASE; PROTEIN KINASE; PROTEIN CRYSTALLIZATION; PROTEIN PURIFICATION;

D O I：

10.1006/jmbi.1993.1248

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The major transitions of the eukaryotic cell cycle are triggered by cyclin-dependent protein kinases. We report the purification and crystallization of the catalytic subunit of human cyclin-dependent kinase 2 (CDK2), which has been implicated in the control of the G1/S transition. CDK2 was purified in large quantities from insect cells infected with a recombinant baculovirus, and crystals of the protein were prepared and subjected to preliminary X-ray diffraction analysis. © 1993 Academic Press, Inc.

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页码：1317 / 1319

页数：3

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