PROTEIN-PHOSPHORYLATION IN RAT CAUDATE HOMOGENATE - STIMULATORY EFFECTS OF DOPAMINE AND ENHANCEMENT OF DOPAMINE RESPONSE FOLLOWING 6-HYDROXYDOPAMINE TREATMENT

被引：8

作者：

HULLIHAN, JP ^{[1
]}

KINNIER, WJ ^{[1
]}

WILSON, JE ^{[1
]}

WILLIAMS, M ^{[1
]}

机构：

[1] UNIV N CAROLINA,DEPT BIOCHEM,CHAPEL HILL,NC 27514

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 583卷 / 02期

关键词：

6-Hydroxydopamine; Cyclic AMP; Dopamine; Protein phosphorylation;

D O I：

10.1016/0304-4165(79)90430-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dopamine (3-hydroxytyramine) stimulates the incorporation of 32P into proteins endogenous to a homogenate of rat caudate nucleus when 10 μM [γ-32P]-ATP is used as a substrate following preincubation with 400 μM ATP. The increase in 32P incorporation has pharmacological characteristics similar to those observed for the dopamines-sensitive adenylate cyclase system present in caudate tissue. Chronic depletion of striatal dopamine in vivo by stereotaxic injection of 6-hydroxydopamine in the nigrostriatal pathway results in a significant enhancement of the dopamine stimulation of 32P incorporation in vitro. Cyclic AMP-stimulated phosphorylation of caudate proteins remains unchanged following 6-hydroxydopamine treatment. © 1979.

引用

页码：232 / 240

页数：9

共 29 条

[1]

ADAMS H, 1963, METHOD ENZYMAT AN, P539

[2] DOPAMINE, A MODULATOR OF CARBOHYDRATE-METABOLISM IN CAUDATE-NUCLEUS [J].