TECHNIQUES OF CAPILLARY LIQUID-CHROMATOGRAPHY

Packed microacapillary columns of the reversed-phase type for liquid chromatography are shown to provide the high efficiencies needed to resolve complex mixtures. Certain demands are placed on the instrumental design for this type of chromatography are shown to provide the high efficiencies needed to resolve complex mixtures. Certain demands are placed on the instrumental design for this type of chromatography, with respect to small volumes of sampling and detection. A direct sampling method is demonstrated that compares favorably with the splitting injection. Two step-wise gradient elution techniques have been developed for work with packed microcapillaries. Complex mixtures of polyaromatic compounds are resolved into numerous fractions detectable with small-volume ultraviolet and spectrofluorometric detectors. © 1979.