PRODUCTION OF CELLULOLYTIC ENZYMES FROM THE XYLARIA AND HYPOXYLON SPECIES OF XYLARIACEAE

被引：21

作者：

WEI, DL

CHANG, SC

WEI, YH

LIN, YW

CHUANG, CL

JONG, SC

机构：

[1] Division of Biology, National Yang-Ming Medical College, Taipei

[2] Department of Biochemistry, National Yang-Ming Medical College, Taipei

[3] Department of Mycology, American Type Culture Collection, Rockville, 20852, Maryland, Parklawn Drive

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1992年 / 8卷 / 02期

关键词：

AVICELASE (EXOCELLULASE); CMCASE (ENDOCELLULASE); BETA-GLUCOSIDASE; XYLAIRACEOUS FUNGI;

D O I：

10.1007/BF01195834

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Eighteen strains of xylariaceous fungi have been screened for higher activities of cellulolytic enzymes, Trichoderma reesei QM 9414 was also examined for comparison. Strains of Xylaria anisopleura and X. regalis had higher endocellulase (CMCase) and exocellulase (Avicelase) activities after 2 weeks' incubation. Hypoxylon stygium produced the highest.activity of beta-glucosidase 3 days after inoculation. The optimum pH for these cellulolytic enzymes was approx. 5.0 and the optimum temperatures ranged from 37 to 50-degrees-C. A mixed culture process using T. reesei QM 9414 and H. stygium was developed to obtain enhanced synthesis of cellulase. Beta-glucosidase activities in the mixed culture increased within 48 h when H. stygium was introduced after 24 h.

引用

页码：141 / 146

页数：6

共 19 条

[1]

Beldman G., Voragen A.G.J., Rombouts F.M., Pilnik W., Synergism in cellulose hydrolysis by endoglucanases and exoglucanases purified from Trichoderma viride, Biotechnology and Bioengineering, 31, pp. 173-178, (1988)

[2]

Bergmeyer H.U., Determination of ‘reducing sugars’ in the cellulose hydrolysate, Methods of Enzymatic Analysis, pp. 26-29, (1984)

[3]

Bisaria V.S., Ghose T.K., Biodegradation of cellulosic materials: substrates, microorganisms, enzymes and products, Enzyme Microbiology and Technology, 3, pp. 90-104, (1981)

[4]

Coutts A.D., Smith R.E., Factors influencing the production of cellulases by Sporotrichum thermophile, Applied and Environmental Microbiology, 31, pp. 819-825, (1976)

[5]

Ghose T.K., Panda T., Bisaria V.S., Effect of culture phasing and mannanase on production of cellulase and hemicellulase by mixed culture of Trichoderma reesei D1-6 and Aspergillus wentii Pt 2804, Biotechnology and Bioengineering, 27, pp. 1353-1361, (1985)

[6]

Hakansson U.L.F., Fagerstam L.G., Pettersson L.G., Andersson L., A 1,4-β-glucan glucanohydrolase from the cellulolytic fungus Trichoderma viride QM 9414, Biochemical Journal, 179, pp. 141-149, (1979)

[7]

Halliwell G., Lovelady J., Utilization of carboxymethyl-cellulose and enzyme synthesis by Trichoderma koningii, Journal of General Microbiology, 126, pp. 211-217, (1981)

[8]

Johnson E.A., Sakajoh M., Halliwell G., Madia A., Demain A.L., Saccarification of complex cellulosic substrates by the cellulase system from Clostridium thermocellum, Applied and Environmental Microbiology, 43, pp. 1125-1132, (1982)

[9]

Ju Y.M., Tzean S.S., Investigations of Xylariaceae in Taiwan 1. The teleomorph of Hypoxylon, Transactions of the Mycological Society of Republic of China, 1, pp. 13-27, (1985)

[10]

Ju Y.M., Tzean S.S., Investigations of Xylariaceae in Taiwan II. The teleomorph of Xylaria, Transactions of The Mycological Society of Republic of China, 1, pp. 103-128, (1985)

← 1 2 →