CONTRACTION-INDUCED TRANSLOCATION OF THE GLUCOSE TRANSPORTER GLUT4 IN ISOLATED VENTRICULAR CARDIOMYOCYTES

被引：51

作者：

KOLTER, T ^{[1
]}

UPHUES, I ^{[1
]}

WICHELHAUS, A ^{[1
]}

REINAUER, H ^{[1
]}

ECKEL, J ^{[1
]}

机构：

[1] DIABET FORSCHUNGSINST,MOLEC CARDIOL LAB,W-4000 DUSSELDORF 1,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 189卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)92333-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Field stimulation of isolated adult ventricular cardiomyocytes was used to study the effect of contractile activity on 3-0-methylglucose transport and the subcellular distribution of Glut4. Cells contracting at a frequency of 1 Hz for 30 min exhibited unaltered basal and insulin-stimulated rates of glucose transport when compared to resting cells. However, at 5 Hz 3-0-methylglucose transport increased to 224% of control after 5 min. Under these conditions insulin was unable to produce a significant additional stimulation of glucose transport. Immunoblotting with an anti-Glut4 polyclonal antibody showed that both insulin and contraction (5 Hz) increased the amount of Glut4 in a plasma membrane fraction by about 8-fold with a parallel decrease in an intracellular membrane fraction by 60 - 65%. These data suggest the existence of an identical insulin- and contraction-recruitable Glut4 transporter pool in cardiomyocytes. © 1992.

引用

页码：1207 / 1214

页数：8

共 33 条

[1] IDENTIFICATION OF A NOVEL GENE ENCODING AN INSULIN-RESPONSIVE GLUCOSE TRANSPORTER PROTEIN [J].

BIRNBAUM, MJ .

CELL, 1989, 57 (02) :305-315

[2] RELATIONSHIP BETWEEN TRANSPORT OF GLUCOSE AND CATIONS ACROSS CELL-MEMBRANES IN ISOLATED TISSUES .9. ROLE OF CELLULAR CALCIUM IN ACTIVATION OF GLUCOSE-TRANSPORT SYSTEM IN RAT SOLEUS MUSCLE [J].

CLAUSEN, T ;

ELBRINK, J ;

DAHLHANSEN, AB .

BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 375 (02) :292-308

[3]

CUSHMAN SW, 1980, J BIOL CHEM, V255, P4758

[4]

DOUEN AG, 1990, J BIOL CHEM, V265, P13427

[5] G-PROTEIN-MEDIATED REGULATION OF THE INSULIN-RESPONSIVE GLUCOSE TRANSPORTER IN ISOLATED CARDIAC MYOCYTES [J].

ECKEL, J ;

GERLACHESKUCHEN, E ;

REINAUER, H .

BIOCHEMICAL JOURNAL, 1990, 272 (03) :691-696

[6] INSULIN ACTION ON THE GLUCOSE-TRANSPORT SYSTEM IN ISOLATED CARDIOCYTES FROM ADULT-RAT [J].

ECKEL, J ;

PANDALIS, G ;

REINAUER, H .

BIOCHEMICAL JOURNAL, 1983, 212 (02) :385-392

[7] INVOLVEMENT OF HORMONE PROCESSING IN INSULIN-ACTIVATED GLUCOSE-TRANSPORT BY ISOLATED CARDIAC MYOCYTES [J].

ECKEL, J ;

REINAUER, H .

BIOCHEMICAL JOURNAL, 1988, 249 (01) :111-116

[8]

FUKUMOTO H, 1989, J BIOL CHEM, V264, P7776

[9] SKELETAL-MUSCLE PLASMA-MEMBRANE GLUCOSE-TRANSPORT AND GLUCOSE TRANSPORTERS AFTER EXERCISE [J].

GOODYEAR, LJ ;

HIRSHMAN, MF ;

KING, PA ;

HORTON, ED ;

THOMPSON, CM ;

HORTON, ES .

JOURNAL OF APPLIED PHYSIOLOGY, 1990, 68 (01) :193-198

[10] GLUCOSE TRANSPORTER PROTEIN-CONTENT AND GLUCOSE-TRANSPORT CAPACITY IN RAT SKELETAL-MUSCLES [J].

HENRIKSEN, EJ ;

BOUREY, RE ;

RODNICK, KJ ;

KORANYI, L ;

PERMUTT, MA ;

HOLLOSZY, JO .

AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 259 (04) :E593-E598

← 1 2 3 4 →