DETECTION OF IMMUNOGLOBULIN HEAVY-CHAIN GENE REARRANGEMENTS IN HODGKINS-DISEASE USING PCR

Aim-To detect clonal rearrangements of the immunoglobulin (Ig) heavy chain gene in Hodgkin's disease tissue using the polymerase chain reaction (PCR). Methods-DNA extracted from 36 samples of Hodgkin's disease was analysed using PCR and primers from conserved sequences in the variable (V(H)) and joining (J(H)) regions. Results-Clonal rearrangement was detected only in one case. Evidence of clonal immunoglobulin gene rearrangement had been detected previously in this case using conventional Southern blot analysis. Conclusions-The sensitivity of the two techniques is equivalent and clonal Ig heavy chain gene rearrangements are rare in Hodgkin's disease.