IDENTIFICATION AND CHARACTERIZATION OF A TRANSMISSIBLE LINEAR PLASMID FROM RHODOCOCCUS-ERYTHROPOLIS BD2 THAT ENCODES ISOPROPYLBENZENE AND TRICHLOROETHENE CATABOLISM

被引:71
作者
DABROCK, B [1 ]
KESSELER, M [1 ]
AVERHOFF, B [1 ]
GOTTSCHALK, G [1 ]
机构
[1] UNIV GOTTINGEN,INST MIKROBIOL,D-37077 GOTTINGEN,GERMANY
关键词
D O I
10.1128/AEM.60.3.853-860.1994
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Rhodococcus erythropolis BD2, which is able to utilize isopropylbenzene as a sole carbon and energy source, was shown to contain a conjugative linear plasmid, pBD2. The estimated size of pBD2 is 208 to 212 kb. Linear plasmid-deficient strains had lost both the isopropylbenzene degradation and trichloroethene degradation characteristics, as well as the arsenite resistance and mercury resistance phenotypes. Reintroduction of pBD2 restored all four characteristics. Conjugational transfer of pBD2 to a plasmidless mutant of strain BD2 and other R. erythropolis strains occurred at frequencies between 3.5 X 10(-5) and 2.6 x 10(-3) transconjugants per recipient. R. erythropolis BD2 degrades isopropylbenzene via 3-isopropylcatechol and 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate. Both isopropylbenzene-oxidizing and meta-cleavage activities were shown to correspond with the presence of pBD2. Southern hybridizations with DNA encoding the toluene dioxygenase structural genes (todC1C2BA) from Pseudomonas putida F1 revealed homology to linear plasmid DNA. These results indicate that the isopropylbenzene degradation pathway encoded by linear plasmid pBD2 is initiated by an isopropylbenzene dioxygenase analogous to toluene dioxygenase.
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页码:853 / 860
页数:8
相关论文
共 40 条
[2]  
ANDERSON MLM, 1985, NUCLEIC ACID HYBRIDI, P73
[3]   3 DIFFERENT 2,3-DIHYDROXYBIPHENYL-1,2-DIOXYGENASE GENES IN THE GRAM-POSITIVE POLYCHLOROBIPHENYL-DEGRADING BACTERIUM RHODOCOCCUS-GLOBERULUS P6 [J].
ASTURIAS, JA ;
TIMMIS, KN .
JOURNAL OF BACTERIOLOGY, 1993, 175 (15) :4631-4640
[4]   MICROBIAL DEGRADATION OF PHENYLALKANES - 2-PHENYLBUTANE, 3-PHENYLPENTANE, 3-PHENYLDODECANE AND 4-PHENYLHEPTANE [J].
BAGGI, G ;
CATELANI, D ;
TRECCANI, V ;
GALLI, E .
BIOCHEMICAL JOURNAL, 1972, 126 (05) :1091-&
[5]   METABOLISM OF THE HERBICIDE ATRAZINE BY RHODOCOCCUS STRAINS [J].
BEHKI, R ;
TOPP, E ;
DICK, W ;
GERMON, P .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (06) :1955-1959
[6]   PLASMID-MEDIATED NICOTINE DEGRADATION IN ARTHROBACTER-OXIDANS [J].
BRANDSCH, R ;
HINKKANEN, AE ;
DECKER, K .
ARCHIVES OF MICROBIOLOGY, 1982, 132 (01) :26-30
[7]   METABOLISM OF QUATERNARY CARBON-COMPOUNDS - 2,2-DIMETHYLHEPTANE AND TERTBUTYLBENZENE [J].
CATELANI, D ;
COLOMBI, A ;
SORLINI, C ;
TRECCANI, V .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1977, 34 (04) :351-354
[8]  
CLAUS D, 1983, CATALOGUE STRAINS GE
[9]   PLASMID-BORNE RESISTANCE TO ARSENATE, ARSENITE, CADMIUM, AND CHLORAMPHENICOL IN A RHODOCOCCUS SPECIES [J].
DABBS, ER ;
SOLE, GJ .
MOLECULAR & GENERAL GENETICS, 1988, 211 (01) :148-154
[10]   ISOPROPYLBENZENE (CUMENE) - A NEW SUBSTRATE FOR THE ISOLATION OF TRICHLOROETHENE-DEGRADING BACTERIA [J].
DABROCK, B ;
RIEDEL, J ;
BERTRAM, J ;
GOTTSCHALK, G .
ARCHIVES OF MICROBIOLOGY, 1992, 158 (01) :9-13