CHARACTERIZATION AND MAPPING OF THE GENE ENCODING MOUSE PROTEASOME SUBUNIT-DELTA (LMP19)

被引：15

作者：

WOODWARD, EC

MONACO, JJ

机构：

[1] UNIV CINCINNATI,HOWARD HUGHES MED INST,DEPT MOLEC GENET,CINCINNATI,OH 45267

[2] VIRGINIA COMMONWEALTH UNIV,DEPT MICROBIOL & IMMUNOL,RICHMOND,VA 23298

来源：

IMMUNOGENETICS | 1995年 / 42卷 / 01期

关键词：

D O I：

10.1007/BF00164984

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The proteasome subunit DELTA is unusually closely related to the major histocompatibility complex (MHC)-linked proteasome subunit, LMP2. The sequence of a mouse cDNA for DELTA confirms that this 22100 M(r) proteasome subunit is highly conserved across species, Sequence analysis of the mouse gene encoding DELTA, designated Lmp19, indicates that it consists of six exons and five introns, similar to the Lmp2 gene. The 5' upstream region lacks a TATA regulatory sequence, which is also absent from proteasome genes isolated from Drosophila. BXD recombinant inbred (RI) mice were used to map the potential chromosomal location of Lmp19, and revealed that the DELTA subunit has related sequences present on two different mouse chromosomes, chromosomes 1 and 11. Typing of 89 progeny from a C57BL/6J X Mus spretus DNA backcross panel (BSS) confirmed the chromosome 1 assignment. Southern hybridization with a polymerase chain reaction-generated Lmpl9 intron 2-specific probe indicates that the Lmpl9 genomic clone corresponds to the sequence on chromosome 11, and further suggests that the chromosome 1 copy represents a processed pseudogene (Lmp19-ps1).

引用

页码：28 / 34

页数：7

共 39 条

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