ANALYSIS AND CRYSTALLIZATION OF A 25 KDA C-TERMINAL FRAGMENT OF CLONED ELONGATION-FACTOR-TS FROM ESCHERICHIA-COLI

被引：10

作者：

BOGESTRAND, S ^{[1
]}

WIBORG, O ^{[1
]}

THIRUP, S ^{[1
]}

NYBORG, J ^{[1
]}

机构：

[1] AARHUS UNIV,DEPT CHEM,DIV BIOSTRUCT CHEM,DK-8000 AARHUS C,DENMARK

来源：

FEBS LETTERS | 1995年 / 368卷 / 01期

关键词：

PROTEIN BIOSYNTHESIS; NUCLEOTIDE EXCHANGE FACTOR; ELONGATION FACTOR TS; TRYPSIN FRAGMENTS; CRYSTALLIZATION; PSEUDO SYMMETRY; ESCHERICHIA COLI;

D O I：

10.1016/0014-5793(95)00597-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 25 kDa C-terminal tryptic fragment of elongation factor Ts has been purified to homogeneity, Experimental evidence suggests that the 25 kDa C-terminal and the 5.3 kDa N-terminal fragments are structurally independent domains. The N-terminal fragment is shown to be essential for the nucleotide exchange activity. Crystals of the C-terminal fragment belong to space group P2 or P2(1), The diffraction pattern shows a pronounced pseudo-C2 symmetry at low resolution. This pseudo symmetry increases when the crystals are irradiated with X-rays for a few hours.

引用

页码：49 / 54

页数：6

共 44 条

[1]

ALLAN MH, 1992, J AM SOC MASS SPECTR, V13, P18

[2] ORGANIZATION AND NUCLEOTIDE-SEQUENCE OF A NEW RIBOSOMAL OPERON IN ESCHERICHIA-COLI CONTAINING THE GENES FOR RIBOSOMAL-PROTEIN S2 AND ELONGATION-FACTOR TS [J].

AN, G ;

BENDIAK, DS ;

MAMELAK, LA ;

FRIESEN, JD .

NUCLEIC ACIDS RESEARCH, 1981, 9 (16) :4163-4172

[3] STUDIES ON POLYPEPTIDE ELONGATION-FACTORS FROM ESCHERICHIA-COLI .5. PROPERTIES OF VARIOUS COMPLEXES CONTAINING EF-TU AND EF-TS [J].

ARAI, KI ;

KAWAKITA, M ;

KAZIRO, Y .

JOURNAL OF BIOCHEMISTRY, 1974, 76 (02) :293-306

[4] CONFORMATIONAL ALTERATION OF PROTEIN-SYNTHESIS ELONGATION-FACTOR EF-TU BY EF-TS AND BY KIRROMYCIN [J].

BLUMENTHAL, T ;

DOUGLASS, J ;

SMITH, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) :3264-3267

[5] NOVEL DATA ON INTERACTIONS OF ELONGATION FACTOR-TS [J].

BUBUNENKO, MG ;

KIREEVA, ML ;

GUDKOV, AT .

BIOCHIMIE, 1992, 74 (05) :419-425

[6]

CHAU V, 1981, J BIOL CHEM, V256, P5591

[7]

ECCLESTON JF, 1988, J BIOL CHEM, V263, P4668

[8]

ECCLESTON JF, 1984, J BIOL CHEM, V259, P2997

[9] A STEPWISE REACTION YIELDING A COMPLEX BETWEEN A SUPERNATANT FRACTION FROM E COLI GUANOSINE 5'-TRIPHOSPHATE AND AMINOACYL-SRNA [J].

GORDON, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 59 (01) :179-&

[10]

GORDON J, 1969, J BIOL CHEM, V244, P5680

← 1 2 3 4 5 →