EFFECT OF AY-9944 ON STEROL BIOSYNTHESIS IN SUSPENSION CULTURES OF BRAMBLE CELLS

被引：58

作者：

SCHMITT, P

BENVENISTE, P

机构：

[1] Institut de Botanique, Laboratoire de Biochimie Végétale, E.R.A. du C.N.R.S. No 487, 67087 Strasbourg Cedex, 28, rue Goethe

来源：

PHYTOCHEMISTRY | 1979年 / 18卷 / 03期

关键词：

PMR of Δ[!sup]8[!/sup] sterols; Rosaceae; Rubus fruticosus; sterol Biosynthesis inhibitor; Δ[!sup]8[!/sup]→Δ[!sup]7[!/sup] isomerase;

D O I：

10.1016/S0031-9422(00)81885-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bramble suspension cultures normally contain Δ5 sterols (sitosterol, campesterol, and isofucosterol). When the cells were grown in a medium supplemented with AY-9944, their content of Δ5 sterols was greatly decreased and Δ8 sterols accumulated. Six Δ8 sterols, including three new compounds, (24R)-24-ethyl-5α-cholest-8-en-3β-ol, stigmasta-8,Z-24(28)-dien-3β-ol, and 4α-methyl-stigmasta-8,Z-24(28)-dien-3β-ol, were identified. AY-9944 probably inhibited the Δ8→Δ7 isomerase. A stable cell line growing permanently in an AY-supplemented medium was obtained. © 1979.

引用

页码：445 / 450

页数：6

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