EXPRESSION OF ANCHORIN CII MESSENGER-RNA BY CULTURED CHONDROCYTES

被引：3

作者：

CASTAGNOLA, P

CANCEDDA, R

机构：

[1] Istituto Nazionale per la Ricerca sul Cancro, Genova, 16132, viale Benedetto XV

来源：

CYTOTECHNOLOGY | 1991年 / 5卷

关键词：

D O I：

10.1007/BF00736804

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about the expression of the gene coding for its receptor: anchorin CII. The modulation of the anchorin mRNA steady state level in chick embryo chondrocytes at different developmental stages is described here. The anchorin mRNA level was low in dedifferentiated chondrocytes, progressively increased after the cell transfer into suspension (a condition promoting differentiation), reached its maximal value after 4 weeks and decreased after 5 weeks. Therefore anchorin CII mRNA reaches its maximum level in hypertrophic stage II chondrocytes.

引用

页码：41 / 44

页数：4

共 11 条

[1]

Castagnola P., Torella G., Cancedda R., Type X collagen synthesis by cultured chondrocytes derived from the permanent cartilaginous region of chick embryo sternum, Dev. Biol., 123, pp. 332-337, (1987)

[2]

Zanetti N.C., Solursh M., Induction of chondrogenesis in limb mesenchymal cultures by disruption of the actin cytosckeleton, J. Cell Biol., 99, pp. 115-123, (1984)

[3]

West C.M., Lanza R., Rosenbloom J., Lowe M., Holtzer H., Avdvalovic N., fibronectin alters the phenotypic properties of cultured chick embryo chondroblasts, Cell, 17, pp. 491-451, (1979)

[4]

Castagnola P., Moro G., Descalzi-Cancedda F., Cancedda R., Type X collagen synthesis during in vitro development of chick embryo tibial chondrocytes, J. Cell Biol., 102, pp. 2310-2317, (1986)

[5]

Castagnola P., Dozin B., Moro G., Cancedda R., Changes in the expression of collagen genes shows two stages in chondrocyte differentiation in vitro, J. Cell Biol., 106, pp. 461-467, (1988)

[6]

Albelda S.M., Buck C.A., Integrins and other cell adhesion molecules, FASEB J., 4, pp. 2869-2880, (1990)

[7]

von der Mark K., Mollenauer J., Muller P.K., Pfaffle M., Anchorin cII, a type II collagen-binding glycoprotein from chondrocyte membranes, Ann. N. Y. Acad. Sci., 460, pp. 214-223, (1985)

[8]

Pfaffle M., Ruggiero F., Hofmann H., Fernandez M.P., Selmin O., Yamada Y., von der Mark, Biosynthesis, secretion and extracellular localization of anchorin CII, a collagen-binding protein of the calpactin family, EMBO J, 7, 8, pp. 2335-2342, (1988)

[9]

Pfefferkorn E.R., Hunter H.S., Purification and partial chemical analysis of Sindbis virus, Virology, 20, pp. 443-445, (1963)

[10]

Ambesi-Impiombato F.S., Parks L.A., Coon H.G., Culture of hormone-dependent functional epithelial cells from rat thyroids, Proc. Natl. Acad. Sci. USA, 77, pp. 3455-3459, (1980)

← 1 2 →