DEVELOPMENT OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR THE DETECTION OF ANTIBODY TO THE PARASITIC DINOFLAGELLATE AMYLOODINIUM-OCELLATUM IN OREOCHROMIS-AUREUS

被引：19

作者：

SMITH, SA

LEVY, MG

NOGA, EJ

机构：

[1] N CAROLINA STATE UNIV,COLL VET MED,DEPT COMPAN ANIM & SPECIAL SPECIES MED,RALEIGH,NC 27606

[2] N CAROLINA STATE UNIV,COLL VET MED,DEPT MICROBIOL PATHOL & PARASITOL,RALEIGH,NC 27606

来源：

VETERINARY PARASITOLOGY | 1992年 / 42卷 / 1-2期

关键词：

D O I：

10.1016/0304-4017(92)90110-U

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

An enzyme-linked immunosorbent assay (ELISA) using antibody to affinity-purified Oreochromis aureus immunoglobulin and antigens from the parasitic dinoflagellate Amyloodinium ocellatum was developed. The ELISA was then used to evaluate the immune response of the tilapine fish to immunization with the parasite. Fish immunized with antigens of the dinospore stage, either live or sonicated, produced a specific immune response that was detectable by this ELISA. Combinations of serial dilutions of A. ocellatum antigen and fish anti-A. ocellatum serum were examined to determine which dilutions provided optimal differentiation of seropositive from seronegative fish. Fresh and heat-inactivated serum from both seropositive and seronegative fish produced similar results.

引用

页码：145 / 155

页数：11

共 27 条

[1]

Ahne W., 1981, Developments in Biological Standardization, V49, P3

[2] THE IMMUNE-RESPONSE IN IMMUNIZED AND NATURALLY INFECTED RAINBOW-TROUT (SALMO-GAIRDNERI) TO DIPLOSTOMUM-SPATHACEUM AS DETECTED BY ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA) [J].

BORTZ, BM ;

KENNY, GE ;

PAULEY, GB ;

GARCIAORTIGOZA, E ;

ANDERSON, DP .

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 1984, 8 (04) :813-822

[3]

BOWER CE, 1983, BASIC MARINE AQUARIU, P192

[4]

Brown E. M., 1931, P ZOOL SOC LOND, V1, P345

[5]

Busch R. A., 1981, Developments in Biological Standardization, V49, P85

[6]

DESAVIGNY D, 1980, DEV CLIN BIOCH, V1, P272

[7]

DICKERSON HW, 1986, AM J VET RES, V47, P2400

[8]

GHITTINO P, 1980, RIV ITAL PISCIC ITTI, V15, P122

[9]

HAMILTON AJ, 1987, DEV COMP IMMUNOL, V11, P253

[10] THE RELATIONSHIP BETWEEN THE ISOLATION OF BRUCELLA-ABORTUS AND SEROLOGICAL STATUS OF INFECTED, NON-VACCINATED CATTLE [J].

HORNITZKY, M ;

SEARSON, J .

AUSTRALIAN VETERINARY JOURNAL, 1986, 63 (06) :172-174

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