MTT ASSAYS ALLOW QUICK AND RELIABLE MEASUREMENT OF THE RESPONSE OF HUMAN TUMOR-CELLS TO PHOTODYNAMIC THERAPY

被引:109
作者
MERLIN, JL
AZZI, S
LIGNON, D
RAMACCI, C
ZEGHARI, N
GUILLEMIN, F
机构
[1] CTR REG TRANSFUS SANGUINE & HEMATOL, CTR ALEXIS VAUTRIN, F-54511 Vandoeuvre Les Nancy, FRANCE
[2] CTR REG TRANSFUS SANGUINE & HEMATOL, CHIM HEMATOL LAB, F-54511 Vandoeuvre Les Nancy, FRANCE
关键词
D O I
10.1016/0959-8049(92)90542-A
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
MCF-7 and HT-29 cell lines were selected as a reliable model to examine the possible parameters affecting the sensitivity of tumour cells to photodynamic therapy (PDT) using a dye-laser at 630 nm. The chemical composition of haematoporphyrin derivative (HPD) was determined by high-performance liquid chromatography (HPLC) analysis and was in agreement with reported values. MTT assays were performed to assess the time-dependency of PDT and the influence of the output power and light fluence. The results showed a maximal cytotoxicity 48 h after photoirradiation. The output power (1 or 2 W) did not significantly affect the cytoxicity when the fluence was constant (20 J/cm2). However, an increase in fluence (10-40 J/cm2) led to a significant enhancement of cytotoxicity until maximal values were reached (30-40 J/cm2). A further increase in fluence (50 J/cm2) proved to induce a fall-off in cytotoxicity related to the intense photobleaching of HPD.
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页码:1452 / 1458
页数:7
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