Children exposed to alcohol prenatally have been found to be at risk for developmental and behavioral problems; however, the relation between maternal alcohol consumption and other environmental factors has not been fully explored in the literature. In this study, subjects were 44 firstborn infants of women over 30 years of age. Structural equation modeling was used to test the hypothesis that maternal alcohol use during pregnancy was related to infant negative affect. Furthermore, negative affect was assumed to influence the mother-infant relationship. It was hypothesized that mothers of infants exhibiting negative affect would be less positive in interaction and that insecure infant attachment behavior would be more prevalent in these infants. Results of model testing confirmed the hypothesis. In addition, maternal alcohol consumption following pregnancy was not found to influence significantly either maternal interaction or infant attachment behavior.