DEUTERIUM NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY AS A PROBE FOR REVERSED-PHASE LIQUID-CHROMATOGRAPHIC BONDED PHASE SOLVATION .2. AQUEOUS SOLVATION IN METHANOL AND ACETONITRILE BINARY MOBILE PHASES

Using the inversion/recovery method, deuterium (H-2) longitudinal relaxation times, (T1), of deuterium oxide were measured for binary solutions of methanol and D2O and acetonitrile and D2O and for these mixtures in contact with two different reversed-phase liquid chromatographic stationary phases. Changes in the D2O T1 for the solutions in contact with the stationary phase compared to those observed for the solutions alone give a qualitative understanding of the degree of association of water with the stationary phase. The results for two stationary phases with greatly different C18-bonding densities are reported and discussed. The results are also compared and contrasted to our previous work, which examined changes in H-2 T1 for deuterated methanol and acetonitrile in the same binary hydroorganic solutions as well as for these mixtures in contact with the same two stationary phases.