RAPID AND SENSITIVE ASSAY FOR ABSCISIC-ACID USING ETHYL ABSCISATE AS AN INTERNAL STANDARD

被引：63

作者：

QUARRIE, SA

机构：

[1] Plant Breeding Institute, Cambridge, CB2 2LQ, Maris Lane, Trumpington

来源：

ANALYTICAL BIOCHEMISTRY | 1978年 / 87卷 / 01期

关键词：

D O I：

10.1016/0003-2697(78)90579-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A gas-liquid chromatographic (glc) assay for abscisic acid (ABA) is described that is both rapid and highly sensitive. The selectivity and sensitivity of an electroncapture detector are used to reduce the assay procedure to just four steps: extraction, thin-layer chromatography (tle), esterification, and gle. Quantification of the ABA present is by reference to ethyl abscisate added during the assay as an internal standard. The procedure enables samples containing as little as 100 pg of ABA to be assayed. © 1978.

引用

页码：148 / 156

页数：9

共 11 条

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