NEUTRAL LIPID AND FATTY ACID COMPOSITION OF EARTHWORMS (LUMBRICUS TERRESTRIS)

被引:23
作者
CERBULIS, J
TAYLOR, MW
机构
[1] Department of Biochemistry and Microbiology Rutgers, The State University, New Brunswick, 08903, New Jersey
关键词
D O I
10.1007/BF02531007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Earthworms (Lumbricus terrestris) were extracted with chloroform-methanol (2:1) and examined primarily for neutral lipids and fatty acids. TLC showed spots for sterols, hydrocarbons, free fatty acids, phospholipids and pigments but none for glycerides (tri-, di- or mono). Saponification of the crude lipid extract yielded 32% fatty acids, 25% unsaponifiables and 43% unidentified. The lipid contained 3% hydrocarbon and 16% sterols. GLC of the hydrocarbons showed at least 13 components. GLC of the sterol fraction showed peaks corresponding to cholesterol (the major component), γ-sitosterol, β-sitosterol, stigmasterol, campesterol, and ergosterol. GLC showed that at least 38 fatty acids were present, with 18:1, 18:2, 18:0, 20:1 and 17:0 predominanting. © 1969 American Oil Chemists' Society.
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页码:363 / &
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